2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Zinthu zodziwika bwino zomwe zimagwiritsidwa ntchito powerengera kuchuluka kwa ma molekyulu: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Chida ndi zida
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Ponseponse, chiwerengero cha amino acid mu zinthu za Sustar ndi chachikulu kuposa cha zinthu za Zinpro.
Gawo 8 Zotsatira za kugwiritsa ntchito
Zotsatira za magwero osiyanasiyana a mchere wochepa pa ntchito yobereka ndi ubwino wa mazira a nkhuku zoyikira mazira kumapeto kwa nthawi yoyikira mazira
Njira Yopangira
Ukadaulo wa chelation wolunjika
Ukadaulo wopaka utoto wa emulsification
Ukadaulo wopopera ndi kuumitsa wa kupanikizika
Ukadaulo wosungira chinyezi mufiriji ndi m'malo osungira chinyezi
Ukadaulo wapamwamba wowongolera zachilengedwe
Zowonjezera A: Njira Zodziwira Kufalikira kwa Ma Peptidi Ochuluka Mwa Ma Molekyulu
Kugwiritsa ntchito muyezo: GB/T 22492-2008
1 Mfundo Yoyesera:
Zinapezeka pogwiritsa ntchito chromatography yoyezera gel yogwira ntchito bwino kwambiri. Izi zikutanthauza kuti, pogwiritsa ntchito porous filler ngati gawo lokhazikika, kutengera kusiyana kwa kukula kwa molekyulu ya zigawo za zitsanzo zolekanitsa, komwe kunapezeka pa peptide bond ya kutalika kwa ultraviolet absorption kwa 220nm, pogwiritsa ntchito pulogalamu yopangira deta yodzipereka kuti idziwe kufalikira kwa molekyulu ya relative pogwiritsa ntchito gel filtration chromatography (monga, pulogalamu ya GPC), ma chromatogram ndi deta yawo zinakonzedwa, kuwerengedwa kuti zipeze kukula kwa molekyulu ya relative ya soya peptide ndi mtundu wa kugawa.
2. Zothandizira
Madzi oyesera ayenera kukwaniritsa zofunikira za madzi ena mu GB/T6682, kugwiritsa ntchito ma reagents, kupatulapo zinthu zapadera, ndi koyera.
2.1 Zinthu zobwezeretsa zinthu zimaphatikizapo acetonitrile (yopanda chromatographically), trifluoroacetic acid (yopanda chromatographically),
2.2 Zinthu zodziwika bwino zomwe zimagwiritsidwa ntchito powerengera kuchuluka kwa ma molekyulu: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Chida ndi zida
3.1 High Performance Liquid Chromatograph (HPLC): malo ogwirira ntchito a chromatographic kapena integrator yokhala ndi UV detector ndi pulogalamu yokonza deta ya GPC.
3.2 Chida chosefera ndi kuchotsa mpweya m'malo oyenda.
3.3 Ndalama yotsala yamagetsi: mtengo womaliza 0.000 1g.
Masitepe 4 Ogwirira Ntchito
4.1 Mikhalidwe ya chromatographic ndi zoyeserera zosinthira machitidwe (mikhalidwe yofotokozera)
- 4.1.1 Chigawo cha Chromatographic: TSKgelG2000swxl300 mm×7.8 mm (m'mimba mwake wamkati) kapena mizati ina ya gel yamtundu womwewo yokhala ndi magwiridwe ofanana oyenera kudziwa mapuloteni ndi ma peptide.
- 4.1.2 Gawo loyenda: Acetonitrile + madzi + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Kutalika kwa nthawi yozindikira: 220 nm.
- 4.1.4 Kuchuluka kwa madzi: 0.5 mL/mphindi.
- 4.1.5 Nthawi yodziwira: mphindi 30.
- 4.1.6 Kuchuluka kwa jakisoni wa chitsanzo: 20μL.
- 4.1.7 Kutentha kwa mzati: kutentha kwa chipinda.
- 4.1.8 Pofuna kuti dongosolo la chromatographic likwaniritse zofunikira zodziwira, zidanenedwa kuti pansi pa mikhalidwe ya chromatographic yomwe ili pamwambapa, mphamvu ya gel chromatographic column, mwachitsanzo, chiwerengero cha ma plates (N), sichinali chochepera 10000 chowerengedwa potengera nsonga za muyezo wa tripeptide (Glycine-Glycine-Glycine).
- 4.2 Kupanga ma curve ofanana a molekyulu
- Mayankho osiyanasiyana a relative molecular mass peptide standard omwe ali pamwambawa okhala ndi kuchuluka kwa 1 mg / mL adakonzedwa ndi mobile phase matching, osakanizidwa mu gawo linalake, kenako adasefedwa kudzera mu organic phase membrane yokhala ndi kukula kwa 0.2 μm ~ 0.5 μm ndikulowetsedwa mu chitsanzo, kenako ma chromatogram a miyezo adapezeka. Ma curve oyerekeza a relative molecular mass ndi ma equation awo adapezeka pojambula logarithm ya relative molecular mass motsutsana ndi nthawi yosungira kapena ndi linear regression.
4.3 Chitsanzo cha mankhwala
Yesani molondola 10mg ya chitsanzo mu botolo la volumetric la 10mL, onjezerani pang'ono gawo loyenda, kugwedeza kwa ultrasonic kwa mphindi 10, kuti chitsanzocho chisungunuke kwathunthu ndikusakanikirana, kuchepetsedwa ndi gawo loyenda mpaka muyeso, kenako kusefedwa kudzera mu nembanemba ya organic phase yokhala ndi kukula kwa pore ya 0.2μm ~ 0.5μm, ndipo filtrate idasanthulidwa malinga ndi momwe chromatographic conditions ilili mu A.4.1.
- 5. Kuwerengera kwa kugawa kwa maselo
- Pambuyo pofufuza yankho la chitsanzo lomwe lakonzedwa mu 4.3 pansi pa mikhalidwe ya chromatographic ya 4.1, kuchuluka kwa mamolekyulu a chitsanzo ndi kuchuluka kwake kogawa kungapezeke mwa kusintha deta ya chromatographic ya chitsanzocho mu calibration curve 4.2 ndi pulogalamu yokonza deta ya GPC. Kugawa kwa kuchuluka kwa mamolekyulu a ma peptide osiyanasiyana kumatha kuwerengedwa pogwiritsa ntchito njira yosinthira malo oyambira, malinga ndi fomula iyi: X=A/A total×100
- Mu fomula iyi: X - Gawo la kulemera kwa peptide yolemera ya molekyulu mu peptide yonse mu chitsanzo, %;
- A - Malo apamwamba a peptide yolemera kwambiri ya molekyulu;
- Chiwerengero A - chiwerengero cha madera okwera kwambiri a peptide iliyonse ya molekyulu, yowerengedwa mpaka malo amodzi a decimal.
- 6 Kubwerezabwereza
- Kusiyana kwakukulu pakati pa zitsimikiziro ziwiri zodziyimira pawokha zomwe zapezeka pansi pa mikhalidwe yoti zitha kubwerezedwa sikuyenera kupitirira 15% ya avhareji ya masamu a zitsimikiziro ziwirizi.
- Zowonjezera B: Njira Zodziwira Ma Free Amino Acids
- Kugwiritsa ntchito muyezo: Q/320205 KAVN05-2016
- 1.2 Ma Reagent ndi zipangizo
- Asidi ya glacial acetic: yoyera bwino
- Asidi ya Perchloric: 0.0500 mol/L
- Chizindikiro: 0.1% kristalo violet chizindikiro (glacial acetic acid)
- 2. Kudziwa ma amino acid aulere
Zitsanzozo zinaumitsidwa pa 80°C kwa ola limodzi.
Ikani chitsanzocho mu chidebe chouma kuti chizizire mwachilengedwe mpaka kutentha kwa chipinda kapena chizizire mpaka kutentha komwe kungagwiritsidwe ntchito.Yesani pafupifupi 0.1 g ya chitsanzo (cholondola mpaka 0.001 g) mu botolo louma la 250 mL.Pitirizani mwachangu kupita ku sitepe yotsatira kuti mupewe kuyamwa chinyezi chozunguliraOnjezani 25 mL ya glacial acetic acid ndipo sakanizani bwino kwa mphindi zosapitirira 5.Onjezani madontho awiri a chizindikiro cha kristalo violetTitrate ndi yankho la 0.0500 mol / L (±0.001) la titration la perchloric acid mpaka yankho litasintha kuchoka pa utoto wofiirira kupita kumapeto.
Lembani kuchuluka kwa yankho lokhazikika lomwe lagwiritsidwa ntchito.
- Chitani mayeso opanda kanthu nthawi yomweyo.
- 3. Kuwerengera ndi zotsatira
- Kuchuluka kwa amino acid X mu reagent kumafotokozedwa ngati gawo lalikulu (%) ndipo kumawerengedwa motsatira fomula iyi: X = C × (V1-V0) × 0.1445/M × 100%, mu fomula iyi:
- C - Kuchuluka kwa yankho la perchloric acid mu ma moles pa lita imodzi (mol/L)
- V1 - Voliyumu yogwiritsidwa ntchito pogawa zitsanzo ndi yankho la perchloric acid, mu milliliters (mL).
- Vo - Voliyumu yogwiritsidwa ntchito poika titration yopanda kanthu ndi yankho la perchloric acid lokhazikika, mu milliliters (mL);
M - Kulemera kwa chitsanzo, mu magalamu (g).
| 0.1445: Unyinji wa amino acid wofanana ndi 1.00 mL wa yankho la perchloric acid wamba [c (HClO4) = 1.000 mol / L]. | 4.2.3 Cerium sulfate muyezo wa titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, yokonzedwa molingana ndi GB/T601. | |
| Kuvomereza miyezo: Q/70920556 71-2024 | 1. Mfundo yodziwira (Fe monga chitsanzo) | Ma amino acid iron complexes ali ndi kusungunuka kochepa kwambiri mu anhydrous ethanol ndipo ma free metal ions amasungunuka mu anhydrous ethanol, kusiyana kwa kusungunuka pakati pa awiriwa mu anhydrous ethanol kunagwiritsidwa ntchito kudziwa kuchuluka kwa chelation kwa amino acid iron complexes. |
| Mu fomula: V1 - voliyumu ya cerium sulfate muyezo womwe umagwiritsidwa ntchito poyesa yankho, mL; | Anhydrous ethanol; zina zonse ndi zofanana ndi gawo 4.5.2 mu GB/T 27983-2011. | 3. Masitepe owunikira |
| Chitani mayeso awiri nthawi imodzi. Yesani 0.1g ya chitsanzo chouma pa 103±2℃ kwa ola limodzi, molondola kufika pa 0.0001g, onjezerani 100mL ya ethanol yosaphwanyika kuti isungunuke, sakanizani, sakanizani zotsalira zosefera zotsukidwa ndi 100mL ya ethanol yosaphwanyika kwa nthawi zosachepera zitatu, kenako sungani zotsalirazo mu botolo la conical la 250mL, onjezerani 10mL ya sulfuric acid solution malinga ndi gawo 4.5.3 mu GB/T27983-2011, kenako chitani izi malinga ndi gawo 4.5.3 “Tenthetsani kuti musungunuke kenako mulole kuti zizizire” mu GB/T27983-2011. Chitani mayeso opanda kanthu nthawi yomweyo. | 4. Kudziwa kuchuluka kwa chitsulo chonse | 4.1 Mfundo yodziwira ndi yofanana ndi gawo 4.4.1 mu GB/T 21996-2008. |
4.2. Zothandizira ndi Mayankho
| 4.2.1 Asidi wosakaniza: Onjezani 150mL ya sulfuric acid ndi 150mL ya phosphoric acid ku 700mL ya madzi ndikusakaniza bwino. | 4.2.2 Sodium diphenylamine sulfonate solution: 5g/L, yokonzedwa molingana ndi GB/T603. | 4.2.3 Cerium sulfate muyezo wa titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, yokonzedwa molingana ndi GB/T601. | |
| 4.3 Njira zowunikira | Chitani mayeso awiri nthawi imodzi. Yesani 0.1g ya chitsanzo, yolondola kufika pa 020001g, ikani mu botolo la conical la 250mL, onjezerani 10mL ya asidi wosakaniza, mutasungunuka, onjezerani 30ml ya madzi ndi madontho 4 a sodium dianiline sulfonate indicator solution, kenako chitani izi motsatira gawo 4.4.2 mu GB/T21996-2008. Chitani mayeso opanda kanthu nthawi yomweyo. | 4.4 Kuyimira zotsatira | Chiwerengero chonse cha chitsulo X1 cha ma amino acid iron complexes malinga ndi kuchuluka kwa chitsulo, mtengo wake womwe wafotokozedwa mu %, unawerengedwa motsatira fomula (1): |
| X1 = (V-V0)×C×M×10-3×100 | V0 - yankho la cerium sulfate lomwe limagwiritsidwa ntchito pogawa yankho lopanda kanthu, mL; | V0 - yankho la cerium sulfate lomwe limagwiritsidwa ntchito pogawa yankho lopanda kanthu, mL; | C - Kuchuluka kwenikweni kwa yankho la cerium sulfate, mol/L5. Kuwerengera kuchuluka kwa chitsulo mu chelatesKuchuluka kwa chitsulo X2 mu chelate malinga ndi gawo la chitsulo, mtengo wake womwe wafotokozedwa mu %, unawerengedwa motsatira fomula iyi: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Mu fomula: V1 - voliyumu ya cerium sulfate muyezo womwe umagwiritsidwa ntchito poyesa yankho, mL; | V2 - yankho la cerium sulfate lomwe limagwiritsidwa ntchito pogawa yankho lopanda kanthu, mL;nom1 - Kulemera kwa chitsanzo, g. Tengani chiwerengero cha masamu cha zotsatira zotsimikizira zofanana ngati zotsatira zotsimikizira, ndipo kusiyana kwakukulu kwa zotsatira zotsimikizira zofanana sikupitirira 0.3%. | 0.05585 - kulemera kwa chitsulo chachitsulo chomwe chimafotokozedwa mu magalamu ofanana ndi 1.00 mL ya yankho la cerium sulfate wamba C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1 - Kulemera kwa chitsanzo, g. Tengani chiwerengero cha masamu cha zotsatira zotsimikizira zofanana ngati zotsatira zotsimikizira, ndipo kusiyana kwakukulu kwa zotsatira zotsimikizira zofanana sikupitirira 0.3%. | 6. Kuwerengera kwa kuchuluka kwa chelationChiŵerengero cha chelation X3, mtengo wofotokozedwa mu %, X3 = X2/X1 × 100Zowonjezera C: Njira Zodziwira Chiwopsezo cha Zinpro cha Chelation |
Kugwiritsa ntchito muyezo: Q/320205 KAVNO7-2016
1. Zopangira mphamvu ndi zipangizo
a) Asidi ya glacial acetic: yoyera bwino; b) Asidi ya perchloric: 0.0500mol/L; c) Chizindikiro: 0.1% chizindikiro cha kristalo violet (asidi ya glacial acetic)
2. Kudziwa ma amino acid aulere
2.1 Zitsanzozo zinaumitsidwa pa 80°C kwa ola limodzi.
2.2 Ikani chitsanzocho mu chidebe chouma kuti chizizire mwachilengedwe mpaka kutentha kwa chipinda kapena chizizire mpaka kutentha komwe kungagwiritsidwe ntchito.
2.3 Yesani pafupifupi 0.1 g ya chitsanzo (cholondola mpaka 0.001 g) mu botolo louma la 250 mL lozungulira
2.4 Pitirizani mwachangu kupita ku gawo lotsatira kuti mupewe kuyamwa chinyezi chozungulira.
2.5 Onjezani 25mL ya glacial acetic acid ndipo sakanizani bwino kwa mphindi zosapitirira 5.
2.6 Onjezani madontho awiri a chizindikiro cha kristalo violet.
2.7 Titrate ndi yankho la 0.0500mol/L (±0.001) la titration ya perchloric acid mpaka yankho litasintha kuchoka pa utoto wofiirira kupita ku wobiriwira kwa masekondi 15 popanda kusintha mtundu ngati mapeto.
2.8 Lembani kuchuluka kwa yankho lokhazikika lomwe lagwiritsidwa ntchito.
2.9 Chitani mayeso opanda kanthu nthawi yomweyo.
- 3. Kuwerengera ndi zotsatira
- Chikatalani
- Physicochemical parameters
V1 - Voliyumu yogwiritsidwa ntchito pogawa zitsanzo ndi yankho la perchloric acid, mu milliliters (mL).
Vo - Voliyumu yogwiritsidwa ntchito poika titration yopanda kanthu ndi yankho la perchloric acid lokhazikika, mu milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adilesi: Nambala 147 Qingpu Road, Shouan Town, Pujiang County, mzinda wa chengdu, chigawo cha Sichuan, China
Foni: 86-18880477902
Zogulitsa
Michere yosafunikira yachilengedwe
- Michere yachilengedwe
- Chiswahili
- Utumiki wosinthidwa
- Maulalo achangu
Mbiri Yakampani
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Chigujarati | Dinani kuti mufunse mafunso | © Copyright - 2010-2025: Ufulu Wonse Ndiwotetezedwa. | Mamapu a tsamba KUSAKA KWAMBIRI Foni |
| Foni | 86-18880477902 | Chijava | Imelo |
| 8618880477902 | Chitchaina | Chifalansa | |
| Bird | Chitchaina | Chifalansa | Chijeremani Chisipanishi |
| Aquatic animals | Chijapani | Chikorea | Chiarabu Chigiriki |
| Chituruki | Chitaliyana | ||
| Ruminant animal g/head day | January 0.75 | Chiindoneziya Chiafrikaans Chiswidishi |
Chipolishi
- Chibasiki
- Chikatalani
- Physicochemical parameters
Chihindi
Chilao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Chishona
Chibugariya
- Chicebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Chikroatia
Chidatchi
| Application object | Chiurdu Chivietnam | Content in full-value feed (mg/kg) | Efficacy |
| Chigujarati | Chihaiti | Chihausa | Chikinyarwanda Chihmong Chihangare |
| Piglets and fattening pigs | Chiigbo | Chijava | Chikannada Chikhmer Chikurdi |
| Chikyrgyz | Chilatini | ||
| Bird | 300～400 | 45～60 | Chimakedoniya Chimalay Chimalayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Chinorwegian
- Chipashito
- Appearance: brownish-yellow granules
- Physicochemical parameters
Chiserbia
Chisutu
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Chishona
Chisindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Chiswahili
Chitajiki
Chitamil
Chitelugu
Chithai
| Application object | Chiurdu Chivietnam | Content in full-value feed (mg/kg) | Efficacy |
| Chiyidishi | Chiyoruba | Chizulu | Chikinyarwanda Chioriya Chiturkmen |
| Chiyughur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
